PHARMACOLOGICALLY ACTIVE NOVEL DAUER PHEROMONE COMPOUND FOR CONTROLLING AGING AND STRESS AND METHOD FOR ISOLATING AND CHARACTERIZING THESAME

摘要

The present invention discloses a method for effectively isolating and purifying a physiological active dauer pheromone compound having the followi ng structural formula I related to aging and stress of C. elegans, and determination of the structure of such material. First, ethanol extract is prepared by completely concentrating a liquid medium with ethanol after culturing C. elegans on a S . basal liquid medium, and then this ethanol fraction is further extracted with ethy l acetate. Then, impurities are removed from ethyl acetate extract by a silica-gel adsorption chromatography and a pheromone extract is isolated and purified by amine column chromatographic procedures using various solvents as eluent. Finally, a pheromone compound having structural formula I is completely isolated and characterized by size-exclusion HPLC.