| 摘要 |
PROBLEM TO BE SOLVED: To provide an immobilization method, which can immobilize a protein in a sample to a solid phase, and which can quantitatively measure/detect the protein by reducing the effect of an inhibitor coexisting in the sample, in which the protein cannot be easily immobilized by a conventional immobilization method, and to provide a protein quantitative determination method, an immuno-blotting method, and a protein immobilization test liquid, using the same. SOLUTION: The method for immobilizing the protein to the solid phase and the immobilization test liquid for use in the same are characterized in that the protein is brought into contact with the solid phase having a hydrophobic surface, under the coexistence of a lower alcohol, a halogenocarboxylic acid, and/or a long-chain alkyl sulfate. The protein quantitative determination method is characterized in that a protein staining liquid is brought into contact with the solid phase in which the protein is immobilized by the immobilization method, and it is performed on the basis of the color development degree generated by it. The immuno-blotting method is characterized in that the solid phase in which the protein is immobilized by the immobilization method is used. COPYRIGHT: (C)2004,JPO
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