| 摘要 |
This invention provides a novel kit for gene expression analysis that can be universally used independent of the target nucleic acid sequence, and a simple method for gene expression analysis utilizing the same. This method comprises: subjecting a gene to be analyzed to real-time PCR detection using a primer comprising a base sequence specifically hybridizing to the target gene, a primer comprising a base sequence identical to the second base sequence, and the TaqMan(R) probe comprising a base sequence identical or complementary to the first base sequence, wherein the gene to be analyzed is prepared by introducing the first base sequence and the second base sequence, which are nonspecific to the base sequence of the target gene, into the target gene so that the second base sequence is bound to a position closer to the 5' end than the first base sequence.
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