发明名称 |
Vaccinia topoisomerases I-based assays for detection of specific DNA breaks |
摘要 |
Several types of DNA cuts are used as markers of apoptosis for detection of apoptotic cells in situ. The present invention includes a method to detect DNase II type DNA damage, bearing 5'OH using vaccinia topoisomerase I. The present invention also includes a method combining a ligase-based method to detect DNase I type DNA damage and the topoisomerase based method, resulting in simultaneous detection of two specific types of DNA damage in situ.
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申请公布号 |
US2004091859(A1) |
申请公布日期 |
2004.05.13 |
申请号 |
US20020068065 |
申请日期 |
2002.02.06 |
申请人 |
DIDENKO VLADIMIR;BASKIN DAVID |
发明人 |
DIDENKO VLADIMIR;BASKIN DAVID |
分类号 |
C07H21/02;C07H21/04;C12Q1/68;G01N1/30;G01N33/48;(IPC1-7):C12Q1/68 |
主分类号 |
C07H21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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