SURROGATE ANTIBODIES AND METHODS OF PREPARATION AND USE THEREOF

摘要

A process is described for producing surrogate antibody molecules that mimic the structure, stability, and binding characteristics of a natural antibody. Surrogate antibody structure, composition of surrogate antibody libraries, methods of surrogate antibody preparation, and surrogate antibody applications are disclosed. Also disclosed are methods of surrogate antibody structural stabilization and resistance to nucleases. The surrogate antibodies comprise a specificity strand and a stabilization strand. The specificity strand comprises a nucleic acid sequence having a specificity region flanked by a first constant region and a second constant region. The stabilization strand comprises a first stabilization region and a second stabilization region that interacts with the second constant region. They can be used as an effective replacement for natural antibody molecules for testing therapeutic agents and research purposes.