| 摘要 |
The invention provides an assay method for detecting fungal infection of soi l or vegetables by pathogenic fungal species, in particular M. acerina, F. carotae and Pythium species, said method comprising: obtaining a sample of soil or vegetable; treating said sample to lyse fungal cells therein; using an oligonucleotide primer pair, effecting a polymerase chain reaction on DNA released by lysis of the fungal cells; and detecting DNA fragments generated by said polymerase chain reaction; wherein said primer pair comprises an 18- to 24-mer having the ability to hybridize to one of the oligonucleotide sequences of formulae (Ia), (Ib), (IIa), (IIb), (IIIa), (IIIb), (IVa), (IVb) , (Va), (Vb), (VIa), (VIb), (VIIa), (VIIb), (VIIIa), (VIIIb), (IXa), (IXb), (Xa), (Xb), (XIa), (XIb), (XIIa), (XIIb), (XIIIa), (XIIIb), (XIVa) and (XIVb ).
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