DNA BASE-SEQUENCING METHOD BY USING COMPOUNDS HAVING ENERGY TRANSFER FUNCTION

摘要

PROBLEM TO BE SOLVED: To provide compounds usable as a primer or an initiator having two reporters capable of serving as a donor and an acceptor in energy transfer and usable in DNA base sequencing by a chain terminator method, and to provide a method for DNA base sequencing by using the same. SOLUTION: The compounds having two reporters capable of serving as the donor and the acceptor in energy transfer, for example, a fluorescent group together with a 2', 3'-deoxyribonucleotide residue or 3'-deoxyribonucleotide residue. The compounds can serve as a terminator in the chain terminator method. These two reporters are located at a sufficient interval for inducing energy transfer from the donor to the acceptor. The method for DNA base sequencing by the chain terminator method carries out the chain termination reaction by using the terminator. COPYRIGHT: (C)2004,JPO