METHOD FOR DETECTING ENDOCRINE DISRUPTORS USING YEAST TWO-HYBRID SYSTEM CONTAINING hERαGENE AND TRANSCRIPTION CO-ACTIVATOR GENE

摘要

PURPOSE: A method for detecting endocrine disruptors using yeast two-hybrid system containing hERα gene and transcription co-activator gene is provided, thereby easily and rapidly detecting chemicals influencing human endocrine system without using mammals or fishes. CONSTITUTION: A method for detecting endocrine disruptors comprises the steps of: (1) treating yeast two-hybrid system containing hERα(human estrogen receptor alpha) gene and transcription co-activator with testing chemicals; and (2) measuring the expression of a reporter gene introduced in the chromosome of yeast.