| 摘要 |
FIELD: biotechnology, biochemistry, molecular biology, protein engineering. SUBSTANCE: invention can be used for preparing glucose oxidase. Recombinant vector for expression of polypeptide with glucose oxidase activity comprises nucleotide sequence encoding polypeptide or its mutein and having Ser at position Cys near amino acid residue 521, and operatively bound regulatory sequences. Cells are transformed with the constructed vector, in particular, cells Saccharomyces cerevisiae. The recombinant polypeptide with glucose oxidase activity is prepared by culturing transformed cells. Invention provides preparing glucose oxidase from fungi by recombinant method. EFFECT: improved preparing method. 10 cl, 17 dwg, 5 tbl |
