发明名称 |
HYBRIDIZATION METHOD THROUGH PERIODIC HEATING AND COOLING AND POLYNUCLEOTIDE DETECTION APPARATUS USING THE SAME |
摘要 |
PURPOSE: A hybridization method through periodic heating and cooling and a polynucleotide detection apparatus using the same are provided, thereby reducing the time for hybridization and increasing the specificity of hybridization, so that the disorder of target polynucleotide can be rapidly and accurately detected. CONSTITUTION: A hybridization method through periodic heating and cooling comprises the steps of: supplying a probe with a target polynucleotide; and circulating a high temperature hybridization process, wherein the hybridization is carried out at higher temperature than the melting point of the probe and target polynucleotide hybrid, and a low temperature hybridization process wherein the hybridization is carried out at lower temperature than the melting point of the probe and target polynucleotide hybrid two or more times, wherein the high temperature is at least 20 deg. C as higher as the melting point; and the low temperature is at least 5 deg. C as lower as the melting point.
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申请公布号 |
KR20040038445(A) |
申请公布日期 |
2004.05.08 |
申请号 |
KR20020067391 |
申请日期 |
2002.11.01 |
申请人 |
ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE |
发明人 |
KIM, GYU WON;KIM, SEONG JIN;KIM, YUN TAE;LEE, DAE SIK;PARK, SE HO;SHIN, YONG BEOM;YANG, HAE SIK;YOON, HYEON CHEOL;YOON, TAE HWAN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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