发明名称 |
METHOD FOR MODIFYING DDR2(DISCOIDIN DOMAIN RECEPTOR) TYROSINE KINASE ACTIVITY AND DDR2 PROTEIN WITH MODIFIED KINASE ACTIVITY THEREBY |
摘要 |
PURPOSE: A method for modifying DDR2(discoidin domain receptor) tyrosine kinase activity and DDR2 protein having modified kinase activity thereby are provided, thereby treating diseases caused by oxidative stresses and over-activation of DDR2 tyrosine kinase. CONSTITUTION: A method for modifying DDR2(discoidin domain receptor) tyrosine kinase activity comprises the steps of: amplifying cDNA encoding the amino acid sequence containing the DDR2 kinase active site and connecting the amplified sequence to the C-terminal coding region of glutathione-S-transferase(GST) to produce DDR2 kinase-GST fusion gene; cloning the DDR2 kinase-GST fusion gene into an expression vector, expressing the tyrosine kinase active site of DDR2 protein-GST fusion protein in a host cell, and purifying the protein; and adding oxidative stresses to the fusion protein to phosphorylate tyrosine in the tyrosine kinase active site of DDR protein.
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申请公布号 |
KR20040038336(A) |
申请公布日期 |
2004.05.08 |
申请号 |
KR20020067233 |
申请日期 |
2002.10.31 |
申请人 |
JE IL PHARMACEUTICAL CO., LTD.;KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
KIM, JEONG HAK;YANG, BEOM SEOK;YANG, GYEONG MI |
分类号 |
C12N9/12;(IPC1-7):C12N9/12 |
主分类号 |
C12N9/12 |
代理机构 |
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