PRODUCTION OF TRANSGENIC BIRDS USING STAGE X PRIMORDIAL GERM CELLS

摘要

The present invention relates to methods for isolating primordial germ cells (PGCs) from the blastoderm of a stage X avian embryo. The present invention further relates to methods for producing a transgenic bird by modifying the isolated PGCs, such that the cells incorporate at least one transgene into their genetic material; transferring the modified PGCs into a suitable recipient, such as a blastoderm of an avian embryo, hatching the embryo; and testing for the presence of the transgene or expression of the protein encoded by the transgene.