发明名称 |
Real-time PCR primers and probes for identification of ralstonia solanacearum race 3, biovar 2 in potato and other plants |
摘要 |
Ralstonia solanacearum, the causal agent of bacterial brown rot of potato, is often carried latently in seed potato tubers. Primers and a probe were designed for a real-time BIO-PCR assay technique for detecting potato tubers latently infected with R. Solanacearum. Using naturally infected potato tubers, as few as 20 cells/ml extract could be detected. Two of 14 naturally infected potato tubers with no disease symptoms were positive by the newly described real-time BIO-PCR (pre-enrichment on agar or in liquid medium) assay but not by direct real-time PCR.
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申请公布号 |
US2004086865(A1) |
申请公布日期 |
2004.05.06 |
申请号 |
US20020283346 |
申请日期 |
2002.10.30 |
申请人 |
SCHAAD NORMAN W.;GAUSH PHILLIP E.;OZAKMAN MERIC |
发明人 |
SCHAAD NORMAN W.;GAUSH PHILLIP E.;OZAKMAN MERIC |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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