METHOD FOR SCREENING FOR MUTANT VIRUS MOVEMENT PROTEINS (MP)

摘要

A method for screening for mutant virus movement proteins (MP) which confer virus resistance to a plant transfected with the mutant MP is provided comprising the steps of (a) carrying out at least one point mutation at a regulatory phosphorylation site of the virus MP, (b) transfecting said mutant MP into at least one plant cell of a cellular structure, (c) analysing an intracellular targeting profile and translocation rate of the mutant MP in the cellular structure and (d) comparing the targeting profile and translocation rate of the mutant MP with the targeting profile and translocation rate of a corresponding wild-type MP, whereby is a mutant virus MP which confers virus resistance to a plant shows localisation to movement relevant cellular structures and a lower translation rate.