发明名称 |
Method of producing fractions containing a high concentration of milk basic cystatin and decomposition products thereof |
摘要 |
A milk basic protein fraction is heated if necessary, the resulting precipitate is removed to recover the resulting supernatant, alcohol is added to this supernatant, a fraction containing a high concentration of milk basic cystatin is recovered by removing the resulting precipitate and brought into contact with a carrier containing a sulfuric acid group, such as heparin, to recover a fraction which is not adsorbed onto the carrier, and this fraction is then treated with an ultrafiltration membrane having an cut-off molecular weight of 10-50 kDa to recover the permeate to produce a fraction containing a high concentration of milk basic cystatin. Alternatively, a milk-derived basic protein composition is brought into contact with an anion exchange resin to recover a fraction which is not adsorbed onto the resin, after which this fraction is brought into contact with a cation exchange resin to recover the target fraction by eluting a fraction which is adsorbed onto the resin with an eluent to produce a fraction containing a high concentration of milk basic cystatin. Further, a decomposition product of the fraction containing a high concentration of milk basic cystatin is produced by decomposing this fraction containing a high concentration of milk basic cystatin with protease. |
申请公布号 |
EP1161881(A3) |
申请公布日期 |
2004.05.06 |
申请号 |
EP20010112980 |
申请日期 |
2001.06.08 |
申请人 |
SNOW BRAND MILK PRODUCTS, CO., LTD. |
发明人 |
TAKADA, YUKIHIRO;SERIZAWA, ATSUSHI;MATSUOKA, YASUHIRO;TOBA, YASUHIRO;KAWAKAMI, HIROSHI |
分类号 |
A21D2/26;A21D13/08;A23C9/13;A23C19/082;A23J1/20;A23J3/08;A23J3/34;A23K1/16;A23K1/175;A23L1/305;A61K35/20 |
主分类号 |
A21D2/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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