Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues

摘要

A method for producing pluripotent (ES) cells that can be used to produce differentiated cells and tissues by: (a)obtaining a haploid cell in metaphase II that comprises DNA derived from a single individual male or female (may be genetically modified); (b)activating the haploid cell by: (1) conditions that do not result in second polar body extrusion; (2) conditions that provide for polar body extrusion but in the presence of an agent that inhibits polar body extrusion, and (3) conditions that prevent the initial cleavage, and culturing said activated cell to produce a gynogenetic or androgenetic embryo comprising a discernible trophectoderm and an inner cell mass; (c)isolating said inner cell mass or cells therefrom and transferring said inner cell mass or cells to an in vitro media that inhibits differentiation of said inner cell mass derived therefrom; and (d)culturing said inner cell mass cells or cells derived therefrom to maintain said cells in an undifferentiated pluripotent state.