| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a means which obtains≥10<SP>8</SP>mesenchymal stem cells per case of a disease necessary for regeneration treatment of mesenchymal tissue without imposing a burden on a patient and selectively grows the mesenchymal stem cells, a means for separating the mesenchymal stem cells and a means for improving efficiency of growth technology. <P>SOLUTION: The method comprises obtaining the mesenchymal stem cells which are fibroblasts settling on a bed floor and growing when a floating fat cell group is separated from a cell-type mixed group obtained by enzyme treatment of a small amount of a fat piece collected from human by centrifugal separation and allowing the fat cell group to stand in a state in which the fat cell group is in contact with the ceiling surface of an incubator charged with a culture solution. The mesenchymal stem cells are grown by subculture and are differentiated into at least bone, cartilage and fat cells. <P>COPYRIGHT: (C)2004,JPO</p> |
