发明名称 |
Compositions and methods to stack multiple nucleotide sequences of interest in the genome of a plant |
摘要 |
Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome. |
申请公布号 |
US2004083500(A1) |
申请公布日期 |
2004.04.29 |
申请号 |
US20030639751 |
申请日期 |
2003.08.12 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
BASZCZYNSKI CHRISTOPHER L.;BOWEN BENJAMIN A.;PETERSON DAVID J.;TAGLIANI LAURA A. |
分类号 |
C12N9/00;C12N15/10;C12N15/11;C12N15/113;C12N15/29;C12N15/31;C12N15/63;C12N15/82;C12N15/90;(IPC1-7):A01H1/00 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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