发明名称 |
DETERMINATION OF HYDROPHOBIC COENZYME A ESTERS AND OTHER LIPIDS USING A BIOSENS OR COMPRISING A MODIFIED COENZYME A- AND ACYL- BINDING PROTEIN (ACBP) |
摘要 |
The invention relates to a biochemical assay for wide class of hydrophobic Coenzyme. A esters wherein the analyte is caused to react with a specifically binding, modified protein, and thereby causing a detectable signal. A one step assay for hydrophobic carboxylic acid esters in whole blood, serum, food and feed preparations, tissue extracts, acyl-CoA synthetase reaction media and various laboratory conditions using a modified Coenzyme A- and acyl-CoA binding protein (ACBP) is provided. Furthermore the invention relates to a construct comprising a peptide and a signal moiety for performing an assay, a kit for assaying hydrophobic CoA esters, hydrophobic carboxylic acids, triacylglycerides, phospholipids, and cholesterolesters.
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申请公布号 |
WO02061096(A8) |
申请公布日期 |
2004.04.29 |
申请号 |
WO2001DK00701 |
申请日期 |
2001.10.24 |
申请人 |
BIOSENSOR APS;KNUDSEN, JENS;WADUM, MAIKEN, CAMILLA, TROLLE;VILLADSEN, JENS;NEERGAARD, THOMAS, B., F. |
发明人 |
KNUDSEN, JENS;WADUM, MAIKEN, CAMILLA, TROLLE;VILLADSEN, JENS;NEERGAARD, THOMAS, B., F. |
分类号 |
C12N15/62;G01N33/573;G01N33/92;(IPC1-7):C12N15/62;C12Q1/00 |
主分类号 |
C12N15/62 |
代理机构 |
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