发明名称 |
Low affinity screening method |
摘要 |
A parallel high throughput screening method on a solid support is disclosed that allows the detection of low affinity binding partners, comprising the steps of:(a) providing a library of different ligands;(b) forming a binding matrix comprising the ligands on a solid support by immobilising said ligands on the support; (c) contacting a target of interest with said binding matrix; (d) parallely determining a binding value of the ligand/target interaction for each type of ligand comprised in the binding matrix; (e) selecting those ligands the binding value of which in an immobilised state towards the target exceeds a predetermined threshold; (f) evaluating the affinity of each of the ligands selected in step (e) in a non-immobilised state towards the target; (g) identifying at least one ligand of step (f) as low affinity binding ligand.
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申请公布号 |
US2004082079(A1) |
申请公布日期 |
2004.04.29 |
申请号 |
US20030467297 |
申请日期 |
2003.09.05 |
申请人 |
BESENBRUCH RALF;OTTLEBEN HOLGER;RAU HARALD;FRANK MICHAEL;SEKUL RENATE;VETTER DIRK;METZ GUNTHER;MAIER SABINE |
发明人 |
BESENBRUCH RALF;OTTLEBEN HOLGER;RAU HARALD;FRANK MICHAEL;SEKUL RENATE;VETTER DIRK;METZ GUNTHER;MAIER SABINE |
分类号 |
G01N33/566;C12M1/00;C12M1/34;C12N15/09;C12Q1/68;G01N33/15;G01N33/50;G01N33/53;G01N33/543;G01N33/547;G01N37/00;(IPC1-7):G01N33/53 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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