| 摘要 |
The present invention provides a method in which antibodies against target proteins contained in samples are screened with high efficiency. Samples including protein groups are treated with two-dimensional electrophoresis, the aforementioned protein groups are developed on a two-dimensional electrophoretic gel as an individual spot protein, the spot protein that had been developed is visualized after being transcribed onto a solid phase, then, after isolating each of the spot proteins, it is reacted with a phage antibody library, antibody that bonded to the spot proteins are replicated, an operation wherein said replicated antibody is reacted with the aforementioned spot protein is conducted once or more than twice, and antibodies against specific proteins contained in the samples including protein groups are screened. As samples including protein groups, it is preferable to use a samples wherein cellular subfraction had been separated and purified beforehand and samples wherein posttranslational modification had been removed.
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