VECTOR FOR THE PRODUCTION OF TRANSPLASTOMIC ANGIOSPERM PLANTS

摘要

The invention relates to a vector for the production of transplastomic angiosperm plants. More specifically, the invention relates to a DNA vector which enables the stable insertion and expression of heterologous genes in the genomes of cell plastids from any angiosperm plant. In the aforementioned vector, the genes to be introduced can be inserted in multiple cloning sites which are located in an artificial intergenic region which is obtained by combining the rbcL and atpB operons from plants of different groups (dicotyledons or monocotyledons). The expression cassette is integrated into the genome by means of recombination between the rbcL and atpB border sequences of the vector with the corresponding homologous regions of the plastid genome. More than one gene of interest can be expressed from the rbcL promoter adjacent to the border region which codes for the atpB gene.