| 摘要 |
<p>The present invention refers to the use of at least one osmolyte to heat stabilise at least one enzyme, such as luciferase and/or apyrase, that is used in an in-vitro bio-luminometric assay, such as a sequencing-by-synthesis reaction, especially Pyrose-quencingTM, during the enzymatic reaction. Moreover, the invention refers to a composition and a kit for use in the bioluminometric assay. Hereby, the temperature sensitive enzyme is heat stabilised, whereby the bioluminometric assay may be per-formed at a temperature that is higher and therefore more suitable for the other en-zymes of the assay.</p> |
