| 摘要 |
PURPOSE: Provided are an antioxidant from the pine needle and an isolation process thereof. The isolated antioxidant has excellent activity as a OH scavenger. CONSTITUTION: An antioxidant from the pine needle is isolated by the steps of: extracting 8.5kg of pine needles with methanol for 3 hours; suspending the extract with distilled water and fractioning it with ethylacetate; purifying the ethylacetate fraction by silica gel column chromatography; and purifying a fraction having antioxidation activity by silica gel chromatography using a mixture of dichloromethane and methanol. The antioxidant includes kaempferol 3-O-galactoside 6'-acetate, kaempferol 3-O-galactoside, and isolariciresinol xyloside.
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