发明名称 |
Process for measuring living body components using polypeptides |
摘要 |
<p>A process is provided for measuring an analyte in a sample derived from a living body which comprises: reacting a sample derived from a living body with a reagent, which reagent comprises a combined product of a polypeptide having 3 to 30 acid residues derived from a strong acid and a substance having affinity for the analyte, separating the resulting complex, and determining the amount of the analyte in the sample on the basis of the amount of the complex. A polypeptide is provided which may be represented by the formula: A-(R<1>)m'-B wherein R<1>'s are, the same or different, independently an amino acid residue introducing a strong acid residue derived from a strong acid having a pKa of 3 or lower thereinto by binding to a reactive group of the amino acid residue, said reactive group being selected from a hydroxyl group, an amino group, an imino group and a thiol group; m' is an integer of 3 to 30; A is a hydrogen atom, a protective group of N-terminus or an acid residue derived from the strong acid; and B is a hydroxyl group or a protective group of C-terminus; excluding those polypeptides having 3 to 20 tyrosine sulfate residues. <IMAGE></p> |
申请公布号 |
EP1273591(B1) |
申请公布日期 |
2004.04.28 |
申请号 |
EP20020016791 |
申请日期 |
1996.07.17 |
申请人 |
WAKO PURE CHEMICAL INDUSTRIES, LTD |
发明人 |
IMAJO, NOBUKO;YAMAGATA, YUKARI;KATOH, HIDEO;SATOMURA, SHINJI;NAKAMURA, KENJI |
分类号 |
C07K1/107;C07K7/02;C07K7/06;C07K7/08;(IPC1-7):C07K1/107;C07K14/00;G01N33/68 |
主分类号 |
C07K1/107 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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