发明名称 |
Charge reduction in electrospray mass spectrometry |
摘要 |
The charge state of ions produced by electrospray ionization is reduced in a controlled manner to yield predominantly singly charged ions through reactions with bipolar ions generated using a <210>Po alpha particle source or equivalent. The multiply charged ions generated by the electrospray undergo charge reduction in a charge reduction chamber. The charge-reduced ions are then detected using a commercial orthogonal electrospray TOF mass spectrometer, although the charge reduction chamber can be adapted to virtually any mass analyzer. The results obtained exhibit a signal intensity drop-off with increased oligonucleotide size similar to that observed with MALDI mass spectrometry, yet with the softness of ESI and without the off-line sample purification and pre-separation required by MALDI.
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申请公布号 |
US6727497(B2) |
申请公布日期 |
2004.04.27 |
申请号 |
US20010815929 |
申请日期 |
2001.03.23 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
SCALF MARK A.;SMITH LLOYD M.;WESTPHALL MICHAEL S.;EBELING DANIEL D. |
分类号 |
H01J49/04;(IPC1-7):H01J49/00;B01D59/44 |
主分类号 |
H01J49/04 |
代理机构 |
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主权项 |
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地址 |
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