BASE SEQUENCE OF pbp1 GENE OF CLINICAL ISOLATE OF HELICOBACTER PYLORI, ASSOCIATED WITH RESISTANCE TO AMOXICILLIN OF b-LACTAM-BASED ANTIBIOTIC, VARIATION OF AMINO ACID SEQUENCE BASED THEREON, VARIANT OF HELICOBACTER PYLORI HAVING THE VARIATION, DIAGNOSIS THEREOF AND METHOD FOR UTILIZING THE SAME FOR DEVELOPMENT OF THERAPEUTIC METHOD

摘要

PROBLEM TO BE SOLVED: To clarify the gene variation of pbp1 associated with amoxicillin resistance by elucidating resistance mechanism of amoxicillin-resistant Helicobacter pylori of a clinical isolate, and to utilize the variant of the Helicobacter pylori for medicinal screening utilizing the variant of the Helicobacter pylori, diagnosis of drug-resistant bacteria based on the variation information of the amino acid sequence based on the base sequence of the pbp1 gene, and development of a new antibiotic utilizing the gene sequence information and a therapeutic method such as gene therapy.

SOLUTION: It is shown that the variation in a penicillin-binding module region present in the C-terminal side half of the pbp1 is important for the transformation to the resistant species by inducing the amoxicillin resistance in the Helicobacter pylori in vitro. As a result, the screening of a medicament having clear action mechanism, the diagnosis of the drug-resistant bacteria based on the variation information of the base sequence of the pbp1 gene and the amino acid sequence based thereon, and the development of the therapeutic method such as the new antibiotic and the gene therapy utilizing the gene sequence information can be developed.

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