摘要 |
The present invention relates to methods for isolating and storing, nucleic acid from a sample containing nucleic acid, such as a cell sample or cell lysate. The nucleic acid is isolated on a solid phase medium, which is then dried, and which can be stored efficiently, such as at room temperature, in columns, tubes, and microwell plates having a wide variety of filters and other solid phase media, for extended periods of time, including days, weeks , and months. The invention provides methods for isolating and storing nucleic acid from a sample by applying the sample to a solid phase medium, retaining the cells, lysing the cellular retentate, drying the medium and retaining th e nucleic acid, storing the nucleic acid for extended periods of time at room temperature and humidity, and optionally eluting the nucleic acid. The invention provides methods for storing nucleic acid-containing samples on a wide range of solid phase media in many types of tubes, columns, or multiwel l plates, many of which are commercial available.
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