发明名称 |
Promoter for the functional characterisation of G-protein coupled receptors in the yeast saccharomyces cerevisiae. |
摘要 |
Recombinant DNA fragment (I) contains the promoter (P) of the Saccharomyces cerevisiae gene YNL279W which regulates transcription of functionally linked structural genes. Independent claims are also included for the following: (1) DNA vector that contains (I); (2) recombinant S. cerevisiae that contains (I); (3) method for identifying activators (A) or inhibitors (B) of G protein-coupled receptors (GPCR); (4) method for producing heterologous proteins (II) in S. cerevisiae by expressing a structural gene for (II) under control of (I); and (5) method for identifying constitutively active mutants of GPCR. |
申请公布号 |
ZA200303120(B) |
申请公布日期 |
2004.04.22 |
申请号 |
ZA20030003120 |
申请日期 |
2003.04.23 |
申请人 |
AVENTIS PHARMA DEUTSCHLAND GMBH. |
发明人 |
DETLEF KOZIAN;ALMUT NITSCHE;PAULINE FRAISSIGNES;SABINE GRATZER;EKKEHARD LEBERER |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N15/85;C12P21/02;C12Q1/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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