TRAP-TAGGING: A NOVEL METHOD FOR THE IDENTIFICATION AND PURIFICATION OF RNA-PROTEIN COMPLEXES

摘要

Conventional methods for the isolation and identification of specific RNA-protein complexes are plagued by a number of problems not encountered in genomics or proteomics. Here we describe a two step affinity purification method used to isolate RNA-protein complexes. The TRAP (Tandem RNA Affinity Purification) tag is a dual RNA tagging system that facilitates gentle purification of RNA molecules along with the proteins, RNAs and other small molecules specifically associated with them.