Method for producing heterologous proteins in a homothallic fungus of the sordariaceae family

摘要

The present invention relates to a method for the production of heterologous protein in a filamentous fungus and promoters suitable therefor. The present invention further relates to vectors and host cells as well as a kit and its use. The object of the present invention is to provide a method for the production of heterologous protein in a filamentous fungus which allows efficient production of heterologous protein and wherein any contamination of the product protein by vegetative spores is avoided. According to the invention this object is solved by a method for the production of heterologous protein in a filamentous fungus which comprises (a) the cultivation of a homothallic fungus of the family Sordariaceae, which contains an expression cassette which contains the following elements in functional combination: a promoter active in the fungus of the family Sordariaceae; a heterologous gene and a terminator active in the fungus of the family Sordariaceae, and (b) the harvesting of the protein produced in an inherently known fashion.