发明名称 METHOD FOR DETECTING POLYMORPHISMS IN NUCLEIC ACIDS
摘要 The present invention provides methods for determining the genotype of a nucleic acid at the site of a polymorphism. The methods achieve sensitivities great enough to detect the presence of any difference between the nucleic acids, even single nucleotide polymorphisms. In the methods, the nucleic acid is compared to a reference nucleic acid having a known genotype. The nucleic acids can be of any length, even less than 100 base pairs. In the methods, one or more extra mismatches are introduced into the nucleic acids at or near the site of the polymorphism. The nucleic acids are contacted under conditions in which they are capable of forming a stable four-way complex that can be detected to indicate that the nucleic acids differ in genotype.
申请公布号 WO03066870(A3) 申请公布日期 2004.04.22
申请号 WO2003US03654 申请日期 2003.02.07
申请人 FRESHGENE, INC.;YANG, QINGHONG 发明人 YANG, QINGHONG
分类号 C12Q1/68 主分类号 C12Q1/68
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