| 摘要 |
A single chain T cell receptor (scTCR) comprising an a segment constituted b y a TCR .alpha. chain variable region sequence fused to the N terminus of a TC R .alpha. chain constant region extracellular sequence, a .beta. segment constituted by a TCR .beta. chain variable region fused to the N terminus of a TCR .beta. chain constant region extracellular sequence, and a linker sequen ce linking the C terminus of the .alpha. segment to the N terminus of the .beta . segment, or vice versa, the constant region extracellular sequences of the .alpha.and .beta. segments being linked by a disulfide bond, the length of t he linker sequence and the position of the disulfide bond being such that the variable region sequences of the .alpha. and .beta. segments are mutually orientated substantially as in native .alpha..beta. T cell receptors. Complexes of two or more such scTCRs, and use of the scTCRs in therapy and i n various screening applications are also disclosed.
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