一个与神经系统发育相关的人类新基因NETRIN－G2

摘要

一个与神经系统发育相关的人类新基因NETRIN－G2涉及人类基因，该基因的cDNA全长2428bp，位于染色体9q34，编码的蛋白质为530个氨基酸，大约60KD，该基因在胚胎各时期和成人的大脑中表达，有望开发成为神经系统疾病治疗的基因药物。

主权项

1、一个与神经系统发育相关的人类新基因NETRIN-G2，其特征在于该基因的cDNA的核苷酸序列和编码氨基酸序列如下： ccatg ctgaggccgc gagtcccgcc tgaccccgtc 35gctgcctctc cagggcttct ctgggccgcg cctctgcaga ctgcgcagcc 85atg ctg cat ctg ctg gcg ctc ttc ctg cac tgc ctc cct ctg gcc 130Met Leu His Leu Leu Ala Leu Phe Leu His Cys Leu Pro Leu Ala1 5 10 15tct ggg gac tat gac atc tgc aaa tcc tgg gtg acc aca gat gag 175Ser Gly Asp Tyr Asp Ile Cys Lys Ser Trp Val Thr Thr Asp Glu16 20 25 30ggc ccc acc tgg gag ttc tac gcc tgc cag ccc aag gtg atg cgc 220Gly Pro Thr Trp Glu Phe Tyr Ala Cys Gln Pro Lys Val Met Arg31 35 40 45ctg aag gac tac gtc aag gtg aag gtg gag ccc tca ggc atc aca 265Leu Lys Asp Tyr Val Lys Val Lys Val Glu Pro Ser Gly Ile Thr46 50 55 60tgt gga gac ccc cct gag agg ttc tgc tcc cat gag aat ccc tac 310Cys Gly Asp Pro Pro Glu Arg Phe Cys Ser His Glu Asn Pro Tyr61 65 70 75cta tgc agc aac gag tgt gac gcc tcc aac ccg gac ctg gcc cac 355Leu Cys Ser Asn Glu Cys Asp Ala Ser Asn Pro Asp Leu Ala His76 80 85 90ccg ccc agg ctc atg ttc gac aag gag gag gag ggc ctg gcc acc 400Pro Pro Arg Leu Met Phe Asp Lys Glu Glu Glu Gly Leu Ala Thr91 95 100 105tac tgg cag agc atc acc tgg agc cgc tac ccc agc ccg ctg gaa 445Tyr Trp Gln Ser Ile Thr Trp Ser Arg Tyr Pro Ser Pro Leu Glu106 110 115 120gcc aac atc acc ctt tcg tgg aac aag acc gtg gag ctg acc gac 490Ala Asn Ile Thr Leu Ser Trp Asn Lys Thr Val Glu Leu Thr Asp121 125 130 135gac gtg gtg atg acc ttc gag tac ggc cgg ccc acg gtc atg gtc 535Asp Val Val Met Thr Phe Glu Tyr Gly Arg Pro Thr Val Met Val136 140 145 150ctg gag aag tcc ctg gac aac ggg cgc acc tgg cag ccc tac cag 580Leu Glu Lys Ser Leu Asp Asn Gly Arg Thr Trp Gln Pro Tyr Gln151 155 160 165ttc tac gcc gag gac tgc atg gag gcc ttc ggt atg tcc gcc cgc 625Phe Tyr Ala Glu Asp Cys Met Glu Ala Phe Gly Met Ser Ala Arg166 170 175 180cgg gcc cgc gac atg tca tcc tcc agc gcg cac cgc gtg ctc tgc 670Arg Ala Arg Asp Met Ser Ser Ser Ser Ala His Arg Val Leu Cys181 185 190 195acc gag gag tac tcg cgc tgg gca ggc tcc aag aag gag aag cac 715Thr Glu Glu Tyr Ser Arg Trp Ala Gly Ser Lys Lys Glu Lys His196 200 205 210gtg cgc ttc gag gtg cgg gac cgc ttc gcc atc ttt gcc ggc ccc 760Val Arg Phe Glu Val Arg Asp Arg Phe Ala Ile Phe Ala Gly Pro211 215 220 225gac ctg cgc aac atg gac aac ctc tac acg cgg ctg gag agc gcc 805Asp Leu Arg Asn Met Asp Asn Leu Tyr Thr Arg Leu Glu Ser Ala226 230 235 240aag ggc ctc aag gag ttc ttc acc ctc acc gac ctg cgc atg cgg 850Lys Gly Leu Lys Glu Phe Phe Thr Leu Thr Asp Leu Arg Met Arg241 245 250 255ctg ctg cgc ccg gcg ctg ggc ggc acc tat gtg cag cgg gag aac 895Leu Leu Arg Pro Ala Leu Gly Gly Thr Tyr Val Gln Arg Glu Asn256 260 265 270ctc tac aag tac ttc tac gcc atc tcc aac atc gag gtc atc ggc 940Leu Tyr Lys Tyr Phe Tyr Ala Ile Ser Asn Ile Glu Val Ile Gly271 275 280 285agg tgc aag tgc aac ctg cat gcc aac ctg tgc tcc atg cgc gag 985Arg Cys Lys Cys Asn Leu His Ala Asn Leu Cys Ser Met Arg Glu286 290 295 300ggc agc ctg cag tgc gag tgc gag cac aac acc acc ggc ccc gac 1030Gly Ser Leu Gln Cys Glu Cys Glu His Asn Thr Thr Gly Pro Asp301 305 310 315 tgc ggc aag tgc aag aag aat ttc cgc acc cgg tcc tgg cgg gcc 1075Cys Gly Lys Cys Lys Lys Asn Phe Arg Thr Arg Ser Trp Arg Ala316 320 325 330ggc tcc tac ctg ccg ctg ccc cat ggc tct ccc aac gcc tgt gcc 1120Gly Ser Tyr Leu Pro Leu Pro His Gly Ser Pro Asn Ala Cys Ala331 335 340 345gct gca ggt tcc ttt ggc aac tgc gaa tgc tac ggt cac tcc aac 1165Ala Ala Gly Ser Phe Gly Asn Cys Glu Cys Tyr Gly His Ser Asn346 350 355 360cgc tgc agc tac att gac ttc ctg aat gtg gtg acc tgc gtc agc 1210Arg Cys Ser Tyr Ile Asp Phe Leu Asn Val Val Thr Cys Val Ser361 365 370 375tgc aag cac aac acg cga ggt cag cac tgc cag cac tgc cgg ctg 1255Cys Lys His Asn Thr Arg Gly Gln His Cys Gln His Cys Arg Leu376 380 385 390ggc tac tac cgc aac ggc tcg gca gag ctg gat gat gag aac gtc 1300Gly Tyr Tyr Arg Asn Gly Ser Ala Glu Leu Asp Asp Glu Asn Val391 395 400 405tgc att gag tgt aac tgc aac cag ata ggc tcc gtg cac gac cgg 1345Cys Ile Glu Cys Asn Cys Asn Gln Ile Gly Ser Val His Asp Arg406 410 415 420tgc aac gag acc ggc ttc tgc gag tgc cgc gag ggc gcg gcg ggc 1390Cys Asn Glu Thr Gly Phe Cys Glu Cys Arg Glu Gly Ala Ala Gly421 425 430 435ccc aag tgc gac gac tgc ctc ccc acg cac tac tgg cgc cag ggc 1435Pro Lys Cys Asp Asp Cys Leu Pro Thr His Tyr Trp Arg Gln Gly436 440 445 450tgc tac ccc aac gtg tgc gac gac gac cag ctg ctg tgc cag aac 1480Cys Tyr Pro Asn Val Cys Asp Asp Asp Gln Leu Leu Cys Gln Asn451 455 460 465gga ggc acc tgc ctg cag aac cag cgc tgc gcc tgc ccg cgc ggc 1525Gly Gly Thr Cys Leu Gln Asn Gln Arg Cys Ala Cys Pro Arg Gly466 470 475 480tac acc ggc gtg cgc tgc gag cag ccc cgc tgc gac ccc gcc gac 1570Tyr Thr Gly Val Arg Cys Glu Gln Pro Arg Cys Asp Pro Ala Asp481 485 490 495gat gac ggc ggt ctg gac tgc gac cgc gcg ccc ggg gcc gcc ccg 1615Asp Asp Gly Gly Leu Asp Cys Asp Arg Ala Pro Gly Ala Ala Pro496 500 505 510cgc ccc gcc acc ctg ctc ggc tgc ctg ctg ctg ctg ggg ctg gcc 1660Arg Pro Ala Thr Leu Leu Gly Cys Leu Leu Leu Leu Gly Leu Ala511 515 520 525gcc cgc ctg ggc cgc tga gccccgc ccggaggacg ctccccgcac 1705Ala Arg Leu Gly Arg527 530ccggaggccg ggggtcccgg ggtcccgggg cggggccggc gtccgaggcc 1755gggcggtgag aagggtgcgg cccgaggtgc tcccaggtgc tactcagcag 1805ggccccccgc ccggcccgcg ctcccgcccg cactgccctc cccccgcagc 1855aggggcgcct tgggactccg gtccccgcgc ctgcgatttg gtttcgtttt 1905tcttttgtat tatccgccgc ccagttcctt ttttgtcttt ctctctctct 1955cttttttttt tttttttctg gcggtgagcc agagggtcgg gagaaacgct 2005gctcgcccca caccccgtcc tgcctcccac cacacttaca cacacgggac 2055tgtggccgac accccctggc ctgtgccagg ctcacgggcg gcggcggacc 2105ccgacctcca gttgcctaca attccagtcg ctgacttggt cctgttttct 2155attctttatt tttcctgcaa cccaccagac cccaggcctc accggaggcc 2205cggtgaccac ggaactcacc gtctggggga ggaggagaga aggaaggggt 2255ggggggcctg gaaacttcgt tctgtagaga actatttttg tttgtattca 2305ctgtcccctg caagggggac ggggcgggag cactggtcac cgcgggggcc 2355gatggtggag aatccgagga gtaaagagtt attccagtcg tgctcactgc 2405tgcaaaaaaa aaaaaaaaaa aaaaaaaa 2428