发明名称 |
METHOD FOR OBTAINING A TGF-BETA ENRICHED PROTEIN FRACTION IN ACTIVATED FORM, PROTEIN FRACTION AND THERAPEUTIC APPLICATIONS |
摘要 |
<p>The invention concerns a method for obtaining a highly enriched TGF-beta protein fraction in activated form, from a liquid solution rich in proteins said to be soluble in the aqueous phase of milk and/or of whey, said method comprising the following steps; a) adjusting soluble proteins purified at a concentration between 5 and 30 g/liter of solution; b) precipitating part of the whey proteins by acidic treatment of the solution thus obtained to a pH ranging between 4 and 5.5 and at a temperature ranging between 55° C. and 68° C.; c) carrying out a microfiltration of the treated solution by diafiltration, so as to obtain respectively a microfiltration retentate and a microfiltrate; d) recuperating the microfiltration retentate containing the protein fraction highly enriched in TGF-beta; e) drying the microfiltration retentate which has been subjected to diafiltration to obtain a powder highly enriched in TGF-beta.</p> |
申请公布号 |
EP1409539(A1) |
申请公布日期 |
2004.04.21 |
申请号 |
EP20020764984 |
申请日期 |
2002.07.12 |
申请人 |
PIERRE JOUAN BIOTECHNOLOGIES S.A. |
发明人 |
MAUBOIS, JEAN-LOUIS;FAUQUANT, JACQUES;JOUAN, PIERRE;BOURTOURAULT, MICHEL |
分类号 |
A61K38/00;A23J1/20;A61K35/20;A61K38/18;A61P1/04;A61P17/02;A61P17/06;A61P19/02;A61P19/10;A61P21/04;A61P29/00;A61P35/00;A61P37/02;A61P37/06;B01D61/14;B01D61/16;B01D61/58;C07K14/495;(IPC1-7):C07K14/495 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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