| 摘要 |
Probes comprising an immobilised Phosphatidic Acid attached onto a solid support are described, for example, as shown in formula I and V: (a) the linker consists of aryl, heteroaryl, alkyl with possible heteroatoms and/or unsaturations, preferably chains of (CH2)n, with n=8-20, most preferably n=11; (b) the heteroatom X maybe O, S, or most preferably NH; (c) the functional group (FG) is a carbonyl from a carboxylate (thiolo)ester, or, most preferably an amide; (d) the R-substituent carries an aryl, alkyl group, or a combination, preferably R=CmH2m+1, m=8-20, m=16 optimal; (e) the ion M represents any cation, preferably Na+, NH4+; (f) unsaturations are allowed, such as in an arachidonyl side chain and (g) =solid support with attachment to functional group, where: R=aryl, alkyl group, or a combination, preferably R=CmH2m+1, m=8-20, m=16 is optimal. R3 is P(O) (OM)2; R5=H(PI(3)P); R3=H; R4=P(O)(OM)2; R5=H(PI(4)P); R3=H; R4=H; R5=P(O)(OM)2(PI(5)P); R3=P(O)(OM)2; R4=P(O)(OM)2; R5=H(PI(3,4)P2); R3=P(O)(OM)2; R4=H; R5=P(O)(OM)2(PI(3,5)P2); R3=H; R4=P(O)(OM)2; R5P=P(O)(OM)2(PI(4,5)P2); or R3=P(O)(OM)2; R4=P(O)(OM)2; R5=H(O)(OM)2(PI(3,4,5)P3). M=any cation, preferably Na+, NH4+ *Denotes a stereogenic center. More preferably a stereogenic centre with an R absolute configuration. Linker=aryl, heteroaryl, alkyl with possible heteroatoms and/or unsaturations. Preferably chains of (CH2)n with n=8-20, most preferably n=11. X=O, S, or, most preferably NH. FG=Carbonyl from a carboxylate, thiolo(ester), or, most preferably an amide. Unsaturations are allowed, such as in an arachidonyl side chain.=solid support with attachment to functional group. Use of the probes to identify Phosphatidic Acid binding protein or phosphoinositide binding proteins is also described.
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