| 摘要 |
An optical system and method of exciting and measuring fluorescence on or in samples treated using fluorescent pigments using such an optical system having at least one first laser (1); a mirror (4); a deflection element (7); an optic (8); and a unit (10), which includes mirror (4) and optic (8) mounted fixed in relation to one another. The unit (10) is positioned so it is linearly movable back and forth along the optical axis (5) and is mechanically connected to an oscillating linear drive (11). The optic (8) additionally acts as a collimator and the mirror (4) additionally acts to deflect the collimated light (12) parallel to the optical axis (5). The optical system additionally includes a table (13), movable at least in the direction of the X and Z spatial axes, for receiving sample holders (14) and for aligning the samples (6) in relation to a first focal point (9); an optical arrangement (16) for imaging a second focal point (17); an aperture plate (18) positioned in the second focal point (17); a first spectral filter (19); and a first detector (20) and is distinguished in that it includes a continuous geometrical axis (G), not running through the sample, on which the optical arrangement (16), the second focal point (17), the aperture plate (18), the first spectral filter (19), and the first detector (20)-together with the mirror (4) and the deflection element (7)-are positioned, this geometrical axis (G) being at least partially identical to the optical axis (5) in the region between the mirror (4) and the first detector (20).
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