发明名称 |
CONCATEMERS OF DIFFERENTIALLY EXPRESSED MULTIPLE GENES |
摘要 |
In the present invention are disclosed concatemers of concatenated expression cassettes and vectors that enable the synthesis of such concatemers. The concatemer comprises in the 5' ->3' direction a cassette of nucleotide sequence of the general formula [rs2-SP-PR-X-TR-SP-rs1]n wherein rs1 and rs2 together denote a functional restriction site, SP individually denotes a spacer of at least two nucleotide bases, PR denotes a promoter, capable of functioning in a cell, X denotes an expressible nucleotide sequence, TR denotes a terminator, and SP individually denotes a spacer of at least two nucleotide bases, and n >/= 2, and wherein at least a first cassette is different from a second cassette. The main purpose of these concatemers is the controllable and co-ordinated expression of large numbers of heterologous genes in a single host. Furthermore, the invention relates to a concatemer of cassettes of nucleotide sequences and a method for preparing the concatemers. In a further aspect, the invention relates to transgenic host cells comprising at least one concatemer according to the invention, as well as to a method for preparing the transgenic host cells. Finally, the invention relates to a vector comprising a cassette of nucleotides, a method for preparing said vector, a nucleotide library comprising at least two primary vectors each comprising a cassette of nucleotides, a method for preparing the library.
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申请公布号 |
WO02059296(A8) |
申请公布日期 |
2004.04.15 |
申请号 |
WO2002DK00055 |
申请日期 |
2002.01.25 |
申请人 |
EVOLVA BIOTECH A/S;GOLDSMITH, NEIL;SOERENSEN, ALEXANDRA, M., P., SANTANA;NIELSEN, SOEREN, V., S.;NAESBY, MICHAEL |
发明人 |
GOLDSMITH, NEIL;SOERENSEN, ALEXANDRA, M., P., SANTANA;NIELSEN, SOEREN, V., S.;NAESBY, MICHAEL |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/66;C12N15/70;C12N15/81;C12N15/85;(IPC1-7):C12N15/66;C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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