| 摘要 |
The invention relates to a method for producing novel modular enzyme systems by genetic engineering, which is characterized by a cyclic in vitro gene synthesis and which allows a specific recombination of the individual, gene-encoded modular components to novel enzyme systems. Examples of such modular enzyme systems are the non-ribosomal peptide synthetases (NRPS) or polyketide synthases (PKS) of type 1, that is the amino acid sequence of such an enzyme is characterized by being composed of a repetitive sequence of identical sequence sections or sequence sections that are very similar to one another. Every single repetitive sequence is referred to as a module and every module allows the enzymatic incorporation of a specific substrate into the substance synthesized by the enzyme. The products synthesized by the NRPS and PKS enzymes are often highly valuable as pharmaceuticals, such as the penicillins, vancomycins or erythromycins. The inventive method allows for an effective production of novel genes for modular enzymes, and the gene expression of said novel genes allows for the production of novel substances.
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