| 摘要 |
A polynucleotide (I) that encodes a polypeptide involved in biosynthesis of spiramycin (A) is any of 35 sequences (B), reproduced, variants (B') of (B), or equivalents of (B) and (B') within the degeneracy of the genetic code, is new. Independent claims are also included for the following: (1) polynucleotides (Ia) that hybridize to (I) under stringent conditions; (2) polynucleotides (Ib) that are at least 70% identical with at least 10 consecutive nucleotides (nt) of (I); (3) polypeptide (II) expressed by (I), (Ia) or (Ib); (4) polypeptides (IIa) involved in synthesis of (A) that has any of 48 sequences (C), reproduced, functional equivalents (C') derived from (C) by one or more substitutions, insertions or deletions of amino acids (aa) in (C), and variants of (C) and (C'); (5) polypeptide (IIb) at least 70% identical with at least 10 consecutive aa of (IIa); (6) recombinant DNA (Ic) that contains at least one (I), (Ia) or (Ib); (7) expression vector containing at least one sequence encoding (IIa) or (IIb); (8) expression system comprising vector and host cells able to produce (IIa) or (IIb); (9) host cell containing at least one (I), (Ia), (Ib), (Ic) or the vector of (7); (10) producing (IIa) or (IIb); (11) microorganisms (X) blocked in a stage in the biosynthetic pathway of at least one macrolide (M); (12) the Streptomyces ambofaciens strains CNCM I-2909 or 2911-2917; (13) preparing intermediates (MI) in biosynthesis of (M); (14) preparing a macrolide derivative (MD) by modification of MI; (15) microorganism (Y) that produces spiramycin I (AI) but not spiramycins II and III; (16) the Streptomyces ambofaciens strain CNCM I-2910; (17) preparing (AI); (18) mutant microorganism (Z) that produces an (M) and has a genetic modification in at least one (I), (Ia) or (Ib) and/or overexpresses such sequences; (19) producing (M) by culturing (Z); and (20) polynucleotide (Id) that is the complement of (I), (Ia) or (Ib). |
