SITE-SPECIFIC MUTATION-INTRODUCING METHOD

摘要

PROBLEM TO BE SOLVED: To provide a site-specific mutation-introducing method in which cDNA is used instead of genome DNA and by which many various mutations can thereby be introduced to remarkably simplify the site-specific mutation introduction into cells.

SOLUTION: This targeting vector is characterized by introducing a site-specific mutation into cDNA and then using the mutated cDNA as a part of genome DNA to form the targeting vector. The mutation is introduced into a cell by a homologous recombination using the targeting vector.

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