发明名称 |
Ends-out gene targeting method |
摘要 |
A gene targeting method for use in a host organism whereby the host organism is transfected with an ends-out donor construct. Further transfecting the host organism with two transgenes expressing endonuclease and recombinase enzymes. The endonuclease and recombinase enzymes are used so that homologous recombination occurs between the DNA segment of the donor construct and a selected gene of the host organism, thus forming a host with containing the recombinogenic donor. The progeny of the host organism, which include the recombinogenic donor are then selected.
|
申请公布号 |
US2004068761(A1) |
申请公布日期 |
2004.04.08 |
申请号 |
US20030434668 |
申请日期 |
2003.05.09 |
申请人 |
GOLIC KENT G.;GONG WEI J. |
发明人 |
GOLIC KENT G.;GONG WEI J. |
分类号 |
A01K67/033;C12N15/90;(IPC1-7):A01K67/033;C12P19/34;C12N15/85;C12N5/06 |
主分类号 |
A01K67/033 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|