发明名称 |
METHOD FOR IDENTIFYING MODULATORS OF SULFOTRANSFERASE ACTIVITY |
摘要 |
The instant invention provides methods for identifying agents that modulate an enzymatic activity of a carbohydrate sulfotransferase. The methods generally involve contacting the sulfotransferase, in a reaction solution, with a sulfate donor, a test agent, and a polymeric sulfate acceptor that is readily separated from the reaction solution. Determination of an effect of the test agent on the sulfotransferase is by detecting the amount of sulfate in the polymeric sulfate acceptor that has been separated from the reaction solution. The invention further provides kits for use in carrying out the subject methods. Figure (1) depicts assays for GST-3 and NDST-1 using polymeric substrates. |
申请公布号 |
WO03098184(A3) |
申请公布日期 |
2004.04.08 |
申请号 |
WO2003US14256 |
申请日期 |
2003.05.06 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;BERTOZZI, CAROLYN;VERDUGO, DAWN |
发明人 |
BERTOZZI, CAROLYN;VERDUGO, DAWN |
分类号 |
C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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