| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a cell-adhered culture substrate which does not cause the peeling of cells, when thawed, and can maintain a high cell survival rate and to which the frozen scaffold-dependent animal cells are adhered, and to provide a method for producing the same. <P>SOLUTION: This cell culture substrate is characterized in that the animal cells are frozen together with the culture substrate in a state that the animal cells are adhered to the cell culture region of the culture substrate and further in a state that a culture liquid containing a viscosity-imparting substance and a freezing-protecting agent is left on the culture surface of the substrate in an amount of 10 to 20ηl/cm<SP>2</SP>. The viscosity of the culture liquid is preferably 10 to 100 centipoises at 1 to 37°C, and DMSO (dimethyl sulfoxide) is contained as a component for the freezing-protecting agent. <P>COPYRIGHT: (C)2004,JPO</p> |
