发明名称 |
IDENTIFICATION OF 3-KETOSTEROID 9-ALFA-HYDROXYLASE GENES AND MICROORGANISMS BLOCKED IN 3-KETOSTEROID 9-ALFA-HYDROXYLASE ACTIVITY |
摘要 |
The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence encoding the amino acid sequence of KshA protein or of KshB protein, encoded by nucleotides 499-1695 of SEQ ID NO: 1 or by nucleotides 387-1427 of SEQ ID NO:2, respectively, and functional homologues thereof. The polynucleotides of the invention can be used to construct genetically modified microorganisms blocked in 3-ketosteroid 9alpha-hydroxylase activity, which are useful in the microbial degradation of steroids to accumulate certain steroid products. |
申请公布号 |
WO03070925(A3) |
申请公布日期 |
2004.04.08 |
申请号 |
WO2003EP50025 |
申请日期 |
2003.02.19 |
申请人 |
AKZO NOBEL N.V.;VAN DER GEIZE, ROBERT;VAN DER MEIJDEN, PETER;HESSELS, GERDA;DIJKHUIZEN, LUBBERT |
发明人 |
VAN DER GEIZE, ROBERT;VAN DER MEIJDEN, PETER;HESSELS, GERDA;DIJKHUIZEN, LUBBERT |
分类号 |
C12N15/09;C12N1/21;C12N9/02;C12P33/00;C12P33/18 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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