BIOMOLECULAR KINETICS METHOD USING A FLOW-THROUGH MICROARRAY

摘要

The present invention relates to methods for monitoring interactions between target-molecules and at least one analyte comprising: (a) contacting a plurality of target-molecules, said target-molecules being immobilized on a porous substrate, with a sample, said sample comprising at least one analyte; (b) incubating said target-molecules with said sample under conditions supporting target-molecule/analyte interaction; (c) monitoring said interaction as defined in step (b), said monitoring being in function of time of incubation as defined in step (b); (d) optionally determining at least one interaction parameter; (e) varying said conditions as defined in step (b) by varying a reaction parameter; and; repeating, at least once, steps (c) to (e). The present invention further relates to the uses of said methods as well as to the use of a porous substrate for the preparation of a microarray kit for carrying out said methods.