| 摘要 |
Proposed is a method of preparing mixed disulphides from a protein and a disulphide component. The method is characterized in that the protein, in an inactive, sparingly soluble form, is incubated with a solution of a denaturing agent at a concentration sufficient for denaturation and in the presence of a disulphide component, dissolved and converted into a derivative. The method is suitable for the high-yield preparation of renaturated recombinant proteins from prokaryotes.
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