| 摘要 |
A water-insoluble enzyme comprises the reaction product of a bacterial acylase and a macromolecular compound having a functional group capable of coupling with an enzyme protein without substantially decreasing the activity thereof. The enzyme may be produced by cultivation of Micrococcus glutamicus or Pseudomonas cruciviae. The macromolecular compound may be polyaminostyrene, p-amino-benzyl cellulose, a nitrated copolymer of methacrylic acid and methacrylic acid-3-fluoroanilide or any compound containing aminophenyl, acid anhydride, acid chloride, isocyanate, acid azide or nitro-fluorophenyl groups.ALSO:Racemic amino acids are resolved by acylating the amino group and selectively hydrolysing the acyl group of the L-form with an enzyme which is rendered insoluble by reaction with a macromolecular compound. Acylated amino acids which may be so hydrolysed includ N-acetyl - L - methionine, - L - alanine, - L - valine, - L - leucine, - L - threonine, - L - norvaline and - L - norleucine, and N - chloroacetyl - L - isoleucine, - L - norleucine, - L - serine, - L - alanine, - L - phenylalanine and - L - valine. Other acid groups which may be removed are benzoyl, dichloroacetyl, chloropropionyl and formyl. The O-form remains unattacked and may be recovered and hydrolysed with acid to give the O-form of the amino acid. |
