HIGH EFFICIENCY GERMLINE TRANSFORMATION SYSTEM

摘要

A method for introducing a heterologous nucleic acid of interest into a plant to thereby produce a recombinant plant is carried out by (a) providing a recombinant nucleic acid of interest, the recombinant nucleic acid comprising the heterologous nucleic acid of interest, and preferably including (i) a matrix attachment region (MAR) positioned 5' to the heterologous DNA, (ii) a MAR positioned 3' to the heterologous nucleic acid of interest, or (iii) a MAR positioned 5' to the heterologous nucleic acid of interest and a MAR positioned 3' to the heterologous nucleic acid of interest; (b) providing meristem tissue of the plant of interest; (c) introducing the recombinant nucleic acid of interest into the meristem tissue; and then (d) propagating a recombinant plant from the meristem tissue, preferably by a direct propagation technique.