| 摘要 |
PURPOSE:To obtain a new lipase having resistance to organic solvents, not loosing enzymatic activity and useful for detergents, pulp processing, synthetic reactions, etc. CONSTITUTION:This lipase is capable of hydrolyzing triglyceride used as a substrate into a fatty acid and glycerin and the lipase has 20-60 deg.C reacting temperature, 6-10 reacting pH range and 9.0-9.5 optimum pH and it has higher enzymatic activity in the presence of dodecane, 2-propanol, 1-heptanol, toluene, n-decane, n-octane, 1-octanol, methanol, n-heptane, n-xylene, cyclohexane, chloroform, decanol, acetone, ethanol, dimethyl sulfoxide, benzene or n-hexane than that in water. The activity of the lipase is lowered by 20-50% by treating it at pH 8.0 and <=20 deg.C or at >=60 deg.C for 5-15min and the activity of the lipase is lowered by >=80% by treating it at 70 deg.C for 5-15min. The lipase is obtained from a supernatant of cultured mixture obtained by culturing Pseudomonas aerugincysa LST-03 (FERM-P-14086), etc. |
